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Thermofisher

Q Exactive HF 高分辨质谱仪

应用范围:蛋白质组学、代谢组学、脂质组学、药代动力学、CRO,蛋白抗体测序

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Q EXACTIVE HF 高分辨质谱仪 性能参数

质量范围(全扫)m/z 50–6000 (Scan range: last   mass ≤ 15 × first mass)
母离子筛选50 < m/z ≤ 400          Any isolation widths between 0.4   amu and full scan
400 < m/z ≤ 700       Any   isolation widths between 0.7 amu and full scan
 700 < m/z ≤ 1000    Any isolation   widths between 1.0 amu and full scan
  1000 < m/z ≤ 2000  Any isolation   widths between 2.0 amu and full scan

  2000 < m/z ≤ 2500  Any isolation   widths between 3.0 amu and full scan

 2500 < m/z                No   precursor ion isolation

分辨率15000 at m/z 200 at a scan rate   of 18 Hz
    30000 at m/z 200 at a scan rate of 12 Hz
    45000 at m/z 200 at a scan rate of 10 Hz
    60000 at m/z 200 at a scan rate of 7 Hz
    120000 at m/z 200 at a scan rate of 3 Hz
    240000 at m/z 200 at a scan rate of 1.5 Hz
质量精度<3 ppm with external   calibration (under defined conditions)
    <1 ppm using internal standard, lock masses (under defined conditions)
正负切换One full cycle in < 1 sec   (one full scan positive mode and one full scan
    negative mode at resolution setting of 35000)
动态范围>5000 within a single scan
灵敏度S/N of a flow injection of   Buspirone for the selected mass chromatogram of the [M+H] + ion (m/z =   386.2551):
    Full Scan: 500 fg on column 100:1
    SIM: 50 fg on column 100:1



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